The impact of alcohol consumption on the liver and kidney functions remains a critical area of investigation, particularly concerning oxidative stress-induced damage. This study examines the effects of chronic alcohol exposure on the liver and kidney tissues of adult male Sprague-Dawley rats and evaluates the potential protective role of glutathione, a key antioxidant. Rats were divided into control, alcohol-exposed, and alcohol-exposed with glutathione supplementation groups. Chronic alcohol administration was used to induce oxidative stress, and glutathione was administered to assess its protective effects. Biochemical assays were conducted to measure markers of liver and kidney injury, including serum transaminases, creatinine, and blood urea nitrogen (BUN). Additionally, histopathological examination of liver and kidney tissues was performed to assess structural damage. The results demonstrated significant increase (p<0.05) in liver and kidney injury markers in alcohol-exposed rats, accompanied by histopathological alterations such as hepatocellular necrosis and renal tubular degeneration. However, glutathione supplementation attenuated these changes, reducing oxidative stress and mitigating organ damage. These findings highlight the detrimental effects of alcohol on kidney and liver function and suggest that glutathione may serve as a potential therapeutic agent for protecting against alcohol-induced organ toxicity.