INVESTIGATION ON THE IMPACT OF ALCOHOL ON THE KIDNEY AND LIVER OF ADULT MALE SPRAGUE-DAWLEY RATS USING GLUTATHIONE AS AN ANTIOXIDANT
Keywords:
Sprague-Dawley Rats., Oxidative Stress, Renal Tubular Degeneration, Hepatocellular Necrosis, GlutathioneAbstract
The impact of alcohol consumption on the liver and kidney functions remains a critical area of investigation, particularly concerning oxidative stress-induced damage. This study examines the effects of chronic alcohol exposure on the liver and kidney tissues of adult male Sprague-Dawley rats and evaluates the potential protective role of glutathione, a key antioxidant. Rats were divided into control, alcohol-exposed, and alcohol-exposed with glutathione supplementation groups. Chronic alcohol administration was used to induce oxidative stress, and glutathione was administered to assess its protective effects. Biochemical assays were conducted to measure markers of liver and kidney injury, including serum transaminases, creatinine, and blood urea nitrogen (BUN). Additionally, histopathological examination of liver and kidney tissues was performed to assess structural damage. The results demonstrated significant increase (p<0.05) in liver and kidney injury markers in alcohol-exposed rats, accompanied by histopathological alterations such as hepatocellular necrosis and renal tubular degeneration. However, glutathione supplementation attenuated these changes, reducing oxidative stress and mitigating organ damage. These findings highlight the detrimental effects of alcohol on kidney and liver function and suggest that glutathione may serve as a potential therapeutic agent for protecting against alcohol-induced organ toxicity.
References
Alani, A. K. (2021). Assessment of Indoor Air Quality and Its Health Effects in Erin Osun Community, Osun State, Nigeria (Master's thesis, Kwara State University (Nigeria))
Anwar, S., Alrumaihi, F., Sarwar, T., Babiker, A. Y., Khan, A. A., Prabhu, S. V., & Rahmani, A. H. (2024). Exploring therapeutic potential of catalase: Strategies in disease prevention and management. Biomolecules, 14(6), 697.
Barnett, L. M., and Cummings, B. S. (2018). Nephrotoxicity and renal pathophysiology: a contemporary perspective. Toxicological Sciences, 164(2), 379-390.
Bergasa, N. V. (2021). Alcohol Induced Liver Disease. In Clinical Cases in Hepatology (pp. 255-293). London: Springer London.
Costa-Valle, M. T., Tonieto, B. D., Altknecht, L., Cunha, C. D., Fão, N., Cestonaro, L. V., ... and Arbo, M. D. (2018). Energy drink and alcohol combination leads to kidney and liver alterations in rats. Toxicology and applied pharmacology, 355, 138-146.
Honda, Y., Kessoku, T., Sumida, Y., Kobayashi, T., Kato, T., Ogawa, Y., ... and Nakajima, A. (2017). Efficacy of glutathione for the treatment of nonalcoholic fatty liver disease: An open-label, single-arm, multicenter, pilot study. BMC gastroenterology, 17, 1-8.
Labarrere, C. A., and Kassab, G. S. (2022). Glutathione: A Samsonian life-sustaining small molecule that protects against oxidative stress, ageing and damaging inflammation. Frontiers in nutrition, 9, 1007816.
Maindonald, R., and Jugdoyal, A. (2020). The patient with acute gastrointestinal problems. In Acute Nursing Care (pp. 415-447).Routledge.
Nassir, F., and Ibdah, J. A. (2014). Role of mitochondria in alcoholic liver disease. World journal of gastroenterology: WJG, 20(9), 2136.
Nevzorova, Y. A., Boyer-Diaz, Z., Cubero, F. J., and Gracia-Sancho, J. (2020). Animal models for liver disease–a practical approach for translational research. Journal of hepatology, 73(2), 423-440.
Ratliff, B. B., Abdulmahdi, W., Pawar, R., and Wolin, M. S. (2016). Oxidant mechanisms in renal injury and disease. Antioxidants & redox signaling, 25(3), 119-146.
Rilwanu, M. M. (2021). Assessment of public health risk of heavy metals from contaminated water, soil and edible vegetables in selected areas of Nasarawa State, Nigeria (Master's thesis, Kwara State University (Nigeria))
Roseline, O. A. (2024). Ezea Charity Chinasa, Ibe Chioma Ifeoma, Ifebi Hope Morris, Ikeh Obiageli Eucharia. Antidiabetic Evaluation of Zingiber zerumbet Linn Rhizome (Zingibereceae) Collected from Agulu of Anambra State, Nigeria. Sch Acad J Pharm, 4, 95-103.
Tan, H. K., Yates, E., Lilly, K., and Dhanda, A. D. (2020). Oxidative stress in alcohol-related liver disease. World journal of hepatology, 12(7), 332.
Zhang, P. L., Pancioli, T., Li, W., and Kanaan, H. D. (2020). Electron microscopic findings can support multiple etiologies of nephrotoxicity in renal tubules. Ultrastructural Pathology, 44(4-6), 481-488.
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